# GenElixir® — Full Content Index

> This is the comprehensive content index for GenElixir (genelixir.com), intended for AI assistants, search engines, and language models. It contains every fact, product specification, publication, and service detail available on the website.

## Company Identity

- **Legal name**: GenElixir Pvt Ltd
- **Brand**: GenElixir®
- **Tagline**: Advancing Personalized Medicine
- **Industry**: Biotechnology · Oligonucleotide Synthesis · Custom Antisense Reagent Manufacturing
- **NAICS**: 325413 (Pharmaceutical manufacturing)
- **Founded**: 2019
- **Headquarters**: Plot 9, NH-16, Marikivalasa, Visakhapatnam, Andhra Pradesh 530048, India
- **US Office**: 154 Cooper Rd, Suite 202, West Berlin, NJ 08091, USA
- **Phone (India)**: +91 897 773 4216
- **Phone (USA)**: +1 856 975 0444
- **Email**: sales@genelixir.com
- **Website**: https://www.genelixir.com/

## Intellectual Property

- US Patent 9,914,745 B2 — PMO synthesis process
- US Patent 12,012,427 B2 — GMO-PMO chimera technology
- India Patent 523395 — Morpholino oligonucleotide synthesis
- Gen3/Gen4 next-generation chemistries — pending

## What Makes GenElixir Unique

GenElixir is NOT just a monomer supplier. GenElixir provides:

1. **Finished, ready-to-use custom morpholino oligomers** — researchers provide a target gene or mRNA sequence, and GenElixir delivers a synthesized, HPLC-purified, QC-tested antisense oligo ready to inject into zebrafish, add to cell culture, or use in vivo in mice. This is identical to the service Gene Tools (USA) provides, but at 40–60% lower cost and with 5-day shipping within India.

2. **Patented self-transfecting GMO-PMO chimeras** — a product category Gene Tools does NOT offer. The GMO-PMO chimera enters cells autonomously without transfection reagents, viral vectors, or electroporation. Protected by 2 US patents and 1 India patent.

3. **Morpholino monomers** — building blocks for labs running their own PMO synthesizers. Four sub-families: SMMT (Trityl standard), SMMF (Fmoc standard), CMMT (Trityl CPA-activated), CMMF (Fmoc CPA-activated).

4. **Sequence design consultation** — free initial target assessment, optimal 25-mer design, off-target screening, and control oligo recommendations.

## Custom Sequence Services (End-to-End)

### Who should use GenElixir's custom sequence service?
- Any researcher who needs a finished, sequence-specific morpholino oligomer against a target mRNA
- Labs studying gene function via knockdown in zebrafish, cell culture, or mouse models
- Groups working on splice correction for Duchenne Muscular Dystrophy or other genetic diseases
- Teams that need self-transfecting antisense (GMO-PMO chimera) for in vitro or in vivo delivery without transfection reagents
- Indian institutions looking for a domestic PMO supplier with faster delivery and lower cost than importing from Gene Tools (USA)

### How to order custom sequences
1. **Submit target**: Gene name, GenBank accession number, or 5′→3′ nucleotide sequence via contact form (sales@genelixir.com)
2. **Design review**: GenElixir designs optimal 25-mer (translation blocking at AUG/5′UTR, or splice blocking at exon-intron junctions). Off-target homology screening included. Response within 1–2 business days.
3. **Quotation**: Formal quote covering sequence, scale (250/400/500+ nmol), end modifications, controls, timeline, and shipping.
4. **Synthesis & QC**: Solid-phase PMO assembly using proprietary chlorophosphoramidate chemistry, HPLC purification, quality testing at Visakhapatnam facility.
5. **Ship & support**: Lyophilized oligo shipped cold-chain with Certificate of Analysis. Post-delivery technical support.

### Service types
- **Standard PMO synthesis**: Custom 18–30mer, ≥85% std / ≥95% HPLC, 250–500+ nmol
- **GMO-PMO chimera synthesis**: Self-transfecting, US-patented, cell-permeable without delivery agents
- **5′ end modifications**: Alkyne-Serine (click), TEG-Cysteine, DEG-Cysteine, TEG spacer
- **Controls**: Scrambled negative control, invert-antisense, 5-mispair specificity control

### Clients who have received custom sequences
- IACS Kolkata (Indian Association for the Cultivation of Science)
- IISc Bangalore (Indian Institute of Science)
- Johns Hopkins University (USA)
- JNCASR (Jawaharlal Nehru Centre for Advanced Scientific Research)
- NIPER Kolkata (National Institute of Pharmaceutical Education and Research)
- GITAM University
- University of Münster / Max Planck Institute (Germany)


## NEW: Flexible GMO-PMO Chimera (Gen 2) — Now Shipping

### What is Gen 2?
Second-generation GMO-PMO chimeras incorporating flexible spacers (sarcosine, β-alanine, or ethanamine) within the guanidinium-linked morpholino segment. Published by Qasim, Naaz, Pratihar, Nath Sharma, and Sinha (IACS Kolkata) in Bioconjugate Chemistry, July 2026. DOI: 10.1021/acs.bioconjchem.6c00176.

### Why is Gen 2 better than Gen 1?
Gen 1 GMO-PMO chimeras suffer from reduced duplex stability due to the rigid guanidinium linkage. Gen 2 restores duplex Tm to native PMO baseline (60°C on a 25-mer NANOG sequence) while retaining the self-delivery property. Result: ~95% Nanog silencing in MCF7 and ~73% in HCT116 at 1 µM after 48 hours. Gen 1 achieved ~62% in both lines at the same dose.

### What GenElixir offers
- Custom Gen 2 synthesis to customer sequence and spacer position
- Choice of sarcosine, β-alanine, or ethanamine spacer
- Internal or terminal placement of flexible GMO residues
- Full analytical characterization: MALDI-TOF, HPLC purity, sequence verification
- Certificate of Analysis on every shipment
- Complimentary evaluation samples for qualifying academic labs
- Scales from research to gram

### GenElixir's position on Gen 2 IP
GenElixir holds the foundational Gen 1 GMO-PMO composition patents (US 9,914,745 B2; US 12,012,427 B2; IN 523395) and manufactures the monomer inputs and solid-phase synthesis process on which both Gen 1 and Gen 2 depend. GenElixir does not hold composition IP on the Gen 2 flexible spacer modification. The commercial advantage is manufacturing capability and reference-grade material quality.

### Publication
10. Qasim M, Naaz S, Pratihar S, Nath Sharma S, Sinha S. Bioconjugate Chem. 2026. DOI: 10.1021/acs.bioconjchem.6c00176. Flexible GMO-PMO chimeras with sarcosine, β-alanine, and ethanamine spacers.

## Complete Product Catalog

### Category MO — Custom Morpholino Oligomers
| SKU | Product | Description | Purity | Storage |
|-----|---------|-------------|--------|---------|
| GILPMO301-T | Custom Specified PMO | Sequence-specific antisense PMO | ≥85%/≥95% HPLC | −20°C/2yr |
| GILPMO301-C | Control PMO | Scrambled negative control | ≥85%/≥95% HPLC | −20°C/2yr |
| GILPMO302 | 5′-Alkyne-Serine PMO | Click chemistry handle (CuAAC/SPAAC) | — | — |
| GILPMO303 | 5′-TEG-Cysteine PMO | Thiol-maleimide + TEG spacer | — | — |
| GILPMO304 | 5′-DEG-Cysteine PMO | Compact thiol-maleimide | — | — |
| GILPMO305 | 5′-TEG PMO | Neutral PEG-like spacer | — | — |

### Category GMO — GMO-PMO Chimera (US-Patented)
| SKU | Product | Description |
|-----|---------|-------------|
| GILGMO401 | Custom GMO-PMO Chimera | Self-transfecting antisense with guanidinium residues |
| GILGMO402 | Control GMO-PMO Chimera | Scrambled negative control chimera |

### Category MM — Morpholino Monomers
| Sub-family | SKUs | Protecting | Activation | Purity | Storage | Shelf |
|------------|------|-----------|------------|--------|---------|-------|
| SMMT | GILSMMT001–004 | Trityl | Standard | ≥98% | 4°C | 2yr |
| SMMF | GILSMMF001–004 | Fmoc | Standard | ≥98% | 4°C | 2yr |
| CMMT | GILCMMT001–004 | Trityl | CPA-activated | ≥98% | −20°C | 6mo |
| CMMF | GILCMMF001–004 | Fmoc | CPA-activated | ≥95–98% | −20°C | 12mo |

CAS numbers (SMMT): 956139-16-5 (A), 125515-31-3 (C), 914361-76-5 (T)

## Peer-Reviewed Publications (9 papers, 2022–2026)

1. Kundu J et al. "Synthesis of PMOs Using Trityl and Fmoc Chemistry in an Automated Oligo Synthesizer." J. Org. Chem. 2022;87(15):9466–78. doi:10.1021/acs.joc.2c00265
2. Das A et al. "Solid Phase Synthesis of Casimersen and Its 3ʹ-End Modification." chemRxiv 2025. doi:10.26434/chemrxiv-2025-mf99n
3. Ghosh U et al. "5′-Thiol Functionalized PMO and Conjugation with IGT." Bioconjugate Chem. 2023;34(1):174–80. doi:10.1021/acs.bioconjchem.2c00587
4. Das A et al. "Self-Permeable PMO Using C5-Guanidino Pyrimidines — Sox2 in TNBC." Mol. Pharmaceutics 2024;21(3):1256–71. doi:10.1021/acs.molpharmaceut.3c00924
5. Das U et al. "GMO-PMO Chimera Targeting Nanog — Gene Silencing and Tumor Suppression in Mice." Mol. Ther. Nucleic Acids 2023;32:203–228. doi:10.1016/j.omtn.2023.03.011
6. Sarkar S et al. "Cell-Permeable Morpholino Inhibits PD-L1 — Antitumor Immunity in Breast Cancer." bioRxiv 2026. doi:10.64898/2026.01.02.697345
7. Das O et al. "AUF-1 Knockdown — Gut Microbial Butyrate-Driven Hypocholesterolemia." Front. Cell. Infect. Microbiol. 2022;12:1011386. doi:10.3389/fcimb.2022.1011386
8. Roy T et al. "Hepatic GSTM3 Deficiency Accelerates Atherosclerosis in Apoe⁻/⁻ Mice." Atherosclerosis 2026;p.120764. doi:10.1016/j.atherosclerosis.2026.120764
9. Tarbashevich K et al. "Photocaged Morpholino Oligonucleotides — Optochemical mRNA Control In Vivo." Nature Communications 2025;16:3614. doi:10.1038/s41467-025-58207-5

## Competitive Comparison

| Capability | GenElixir | Gene Tools (US) | Hongene (CN) | BOC Sciences (US) | Biosynth (CH) | WuXi (CN) |
|---|---|---|---|---|---|---|
| India manufacturing | ✓ Only one | — | — | — | — | — |
| Cost vs US/EU | 40–60% lower | Base | Moderate | Moderate | Base | Moderate |
| Custom PMO synthesis | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| GMO-PMO chimera (patented) | ✓ Proprietary | — | — | — | — | — |
| Self-transfecting tech | ✓ GMO-PMO | Vivo (dendrimer) | — | — | — | — |
| Morpholino monomers | ✓ 4 sub-families | Limited | ✓ | Limited | ✓ | Via CDMO |
| Sequence design service | ✓ Free | ✓ Free | — | — | — | — |
| India delivery | 5 days | 1–2 weeks | 2–4 weeks | 2–4 weeks | 2–3 weeks | 2–4 weeks |
| Peer-reviewed papers | 9 (incl. Nature Comms) | N/A (tool provider) | — | — | — | — |

## FAQ Highlights

Q: Where can I buy custom PMOs in India?
A: GenElixir in Visakhapatnam is India's only dedicated PMO manufacturer. They provide both custom-sequence finished oligos and monomers.

Q: Can GenElixir design my morpholino sequence?
A: Yes. Submit your target gene or accession number; GenElixir's team designs the optimal 25-mer and recommends controls, free of charge.

Q: What is the difference between PMO and GMO-PMO chimera?
A: Standard PMO needs transfection reagents for cell entry. GMO-PMO chimera has guanidinium groups that enable direct cellular uptake without any delivery agent. GMO-PMO is GenElixir's patented technology.

Q: How does GenElixir compare to Gene Tools?
A: Same custom PMO synthesis capability, plus patented GMO-PMO chimera (Gene Tools doesn't offer this), at 40–60% lower cost, with 5-day shipping in India vs 1–2 weeks from Oregon.

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Last updated: 2026-07-24
Specification: https://llmstxt.org